Mastitis infection is a prevalent disease in dairy animals characterized by the inflammation of the mammary gland of the infected animal. Mastitis infection affects both quality and quantity of milk thus causing severe economic burden to dairy farmers worldwide. In the Philippines, the rapidly growing population and strong economic growth is pushing further the demand for milk. However, the prevalence of mastitis infection undermines the government’s efforts in improving local dairy industry. The country is hoping to increase local milk production from its current measly levels which only accounts roughly at 1% of the total supply. The common farm practice in addressing mastitis infection is the administration of antibiotic to farm animals. This practice however have multiple negative implications as it results to high volume of milk wastage as well as high risk of residual antibiotic contamination in milk. Antibiotic contamination in food have been shown to pose adverse health effects to humans. A more serious dimension of antibiotic contamination in food is on its linked to the emergence of antibiotic resistance in many bacterial pathogens. Meanwhile, bacteriocins are antimicrobial compounds that have been utilized as a food-grade antimicrobial agent in many industrialized countries. These compounds are inherently potent against mastitis-causing bacterial pathogens such as Streptococcus agalactiae, Corynebacterium bovis, and Staphylococcus aureus. This proposed research undertaking hopes to establish the utility of bacteriocin-based prophylactic and therapeutic formulation against mastitis infection in dairy cattle. The success of this proposed project should benefit dairy farmers by providing access to food-grade alternative for the prevention and control of mastitis infection. This proposed research undertaking is aligned to the government’s efforts for the development of the local dairy industry. The food-grade nature of bacteriocins should also address the problem of residual antibiotic contamination in milk from