Antibiotics have revolutionized medicine in many aspects, and their discovery is considered a turning point in human history. Nevertheless, the major consequence of the use of antibiotics is the contaminant development of resistance against them, thus there is a decline in the number of new antibiotics approved for clinical use. Terrestrial Actinobacteria have severed as a primary source of antibiotics for more than five decades, however, a rapid decrease in the discovery of new effective antibiotics strongly necessitates new approaches or drawing interest to new ecological niche such as marine sediments. Actinobacteria thriving in unexplored marine sediments, represent untapped sources of potentially new bioactive compounds such as antimicrobials. The Philippines is uniquely positioned in the tropics that host very diverse marine organisms and microorganism. The Actinobacteria thriving in the Philippine marine sediments have not been explored for its antibiotic potential, thus we focused our investigation on isolating unique and diverse marine sediment-derived actinobacteria from the Philippines as resource for new antibiotics. We collected marine sediments from eleven Islands (Unisa Island, Nogas Island, Seco Island, Mararison, Island Batbatan Island, Danjugan Island, Apo Island, Sumilon Island, Balicasag Island, Olotayan Island, and Crocodile Island) in Western and Central Visayas, and Isolated marine sediment-derived actinobacteria by culture dependent isolation technique. We now have a library of 2,406 marine sediment derived actinobacteria isolates from Western and Central Visayas.